Genetic transformation of Populus trichocarpa genotype Nisqually-1: a functional genomic tool for woody plants.
Identifieur interne : 003E12 ( Main/Exploration ); précédent : 003E11; suivant : 003E13Genetic transformation of Populus trichocarpa genotype Nisqually-1: a functional genomic tool for woody plants.
Auteurs : Jingyuan Song [République populaire de Chine] ; Shanfa Lu ; Zenn-Zong Chen ; Rodrigo Lourenco ; Vincent L. ChiangSource :
- Plant & cell physiology [ 0032-0781 ] ; 2006.
Descripteurs français
- KwdFr :
- Agrobacterium tumefaciens (MeSH), Bois (génétique), Bois (microbiologie), Bois (physiologie), Glucuronidase (métabolisme), Génome végétal (génétique), Génomique (méthodes), Génotype (MeSH), Populus (génétique), Populus (microbiologie), Populus (physiologie), Régénération (MeSH), Transformation génétique (MeSH), Végétaux génétiquement modifiés (MeSH).
- MESH :
- génétique : Bois, Génome végétal, Populus.
- microbiologie : Bois, Populus.
- métabolisme : Glucuronidase.
- méthodes : Génomique.
- physiologie : Bois, Populus.
- Agrobacterium tumefaciens, Génotype, Régénération, Transformation génétique, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (MeSH), Genome, Plant (genetics), Genomics (methods), Genotype (MeSH), Glucuronidase (metabolism), Plants, Genetically Modified (MeSH), Populus (genetics), Populus (microbiology), Populus (physiology), Regeneration (MeSH), Transformation, Genetic (MeSH), Wood (genetics), Wood (microbiology), Wood (physiology).
- MESH :
- chemical , metabolism : Glucuronidase.
- genetics : Genome, Plant, Populus, Wood.
- methods : Genomics.
- microbiology : Populus, Wood.
- physiology : Populus, Wood.
- Agrobacterium tumefaciens, Genotype, Plants, Genetically Modified, Regeneration, Transformation, Genetic.
Abstract
We report here the Agrobacterium-mediated genetic transformation of Nisqually-1, a Populus trichocarpa genotype whose genome was recently sequenced. Several systems were established. Internodal stem segments from vigorously growing greenhouse plants are the explants most amenable to transformation. For the most efficient system, approximately 40% of the stem segments infected with pBI121-containing Agrobacterium tumefaciens C58 produced transgenic calli, as confirmed by beta-glucuronidase (GUS) staining. The regeneration efficiency of independent transgenic plants was approximately 13%, as revealed by genomic Southern analysis. Some transgenic plants were produced in as little as 5 months after co-cultivation. This system may help to facilitate studies of gene functions in tree growth and development at a genome level.
DOI: 10.1093/pcp/pcl018
PubMed: 17018558
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Genetic transformation of Populus trichocarpa genotype Nisqually-1: a functional genomic tool for woody plants.</title><author><name sortKey="Song, Jingyuan" sort="Song, Jingyuan" uniqKey="Song J" first="Jingyuan" last="Song">Jingyuan Song</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Xibeiwang, Haidian District, Beijing 100094, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Xibeiwang, Haidian District, Beijing 100094</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Lu, Shanfa" sort="Lu, Shanfa" uniqKey="Lu S" first="Shanfa" last="Lu">Shanfa Lu</name></author><author><name sortKey="Chen, Zenn Zong" sort="Chen, Zenn Zong" uniqKey="Chen Z" first="Zenn-Zong" last="Chen">Zenn-Zong Chen</name></author><author><name sortKey="Lourenco, Rodrigo" sort="Lourenco, Rodrigo" uniqKey="Lourenco R" first="Rodrigo" last="Lourenco">Rodrigo Lourenco</name></author><author><name sortKey="Chiang, Vincent L" sort="Chiang, Vincent L" uniqKey="Chiang V" first="Vincent L" last="Chiang">Vincent L. Chiang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:17018558</idno><idno type="pmid">17018558</idno><idno type="doi">10.1093/pcp/pcl018</idno><idno type="wicri:Area/Main/Corpus">003D17</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003D17</idno><idno type="wicri:Area/Main/Curation">003D17</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003D17</idno><idno type="wicri:Area/Main/Exploration">003D17</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Genetic transformation of Populus trichocarpa genotype Nisqually-1: a functional genomic tool for woody plants.</title><author><name sortKey="Song, Jingyuan" sort="Song, Jingyuan" uniqKey="Song J" first="Jingyuan" last="Song">Jingyuan Song</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Xibeiwang, Haidian District, Beijing 100094, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Xibeiwang, Haidian District, Beijing 100094</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Lu, Shanfa" sort="Lu, Shanfa" uniqKey="Lu S" first="Shanfa" last="Lu">Shanfa Lu</name></author><author><name sortKey="Chen, Zenn Zong" sort="Chen, Zenn Zong" uniqKey="Chen Z" first="Zenn-Zong" last="Chen">Zenn-Zong Chen</name></author><author><name sortKey="Lourenco, Rodrigo" sort="Lourenco, Rodrigo" uniqKey="Lourenco R" first="Rodrigo" last="Lourenco">Rodrigo Lourenco</name></author><author><name sortKey="Chiang, Vincent L" sort="Chiang, Vincent L" uniqKey="Chiang V" first="Vincent L" last="Chiang">Vincent L. Chiang</name></author></analytic><series><title level="j">Plant & cell physiology</title><idno type="ISSN">0032-0781</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (MeSH)</term><term>Genome, Plant (genetics)</term><term>Genomics (methods)</term><term>Genotype (MeSH)</term><term>Glucuronidase (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (genetics)</term><term>Populus (microbiology)</term><term>Populus (physiology)</term><term>Regeneration (MeSH)</term><term>Transformation, Genetic (MeSH)</term><term>Wood (genetics)</term><term>Wood (microbiology)</term><term>Wood (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agrobacterium tumefaciens (MeSH)</term><term>Bois (génétique)</term><term>Bois (microbiologie)</term><term>Bois (physiologie)</term><term>Glucuronidase (métabolisme)</term><term>Génome végétal (génétique)</term><term>Génomique (méthodes)</term><term>Génotype (MeSH)</term><term>Populus (génétique)</term><term>Populus (microbiologie)</term><term>Populus (physiologie)</term><term>Régénération (MeSH)</term><term>Transformation génétique (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glucuronidase</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genome, Plant</term><term>Populus</term><term>Wood</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Bois</term><term>Génome végétal</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Genomics</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Bois</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Populus</term><term>Wood</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glucuronidase</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Génomique</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Bois</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Populus</term><term>Wood</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Genotype</term><term>Plants, Genetically Modified</term><term>Regeneration</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Agrobacterium tumefaciens</term><term>Génotype</term><term>Régénération</term><term>Transformation génétique</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We report here the Agrobacterium-mediated genetic transformation of Nisqually-1, a Populus trichocarpa genotype whose genome was recently sequenced. Several systems were established. Internodal stem segments from vigorously growing greenhouse plants are the explants most amenable to transformation. For the most efficient system, approximately 40% of the stem segments infected with pBI121-containing Agrobacterium tumefaciens C58 produced transgenic calli, as confirmed by beta-glucuronidase (GUS) staining. The regeneration efficiency of independent transgenic plants was approximately 13%, as revealed by genomic Southern analysis. Some transgenic plants were produced in as little as 5 months after co-cultivation. This system may help to facilitate studies of gene functions in tree growth and development at a genome level.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17018558</PMID><DateCompleted><Year>2007</Year><Month>02</Month><Day>13</Day></DateCompleted><DateRevised><Year>2011</Year><Month>11</Month><Day>17</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0032-0781</ISSN><JournalIssue CitedMedium="Print"><Volume>47</Volume><Issue>11</Issue><PubDate><Year>2006</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Plant & cell physiology</Title><ISOAbbreviation>Plant Cell Physiol</ISOAbbreviation></Journal><ArticleTitle>Genetic transformation of Populus trichocarpa genotype Nisqually-1: a functional genomic tool for woody plants.</ArticleTitle><Pagination><MedlinePgn>1582-9</MedlinePgn></Pagination><Abstract><AbstractText>We report here the Agrobacterium-mediated genetic transformation of Nisqually-1, a Populus trichocarpa genotype whose genome was recently sequenced. Several systems were established. Internodal stem segments from vigorously growing greenhouse plants are the explants most amenable to transformation. For the most efficient system, approximately 40% of the stem segments infected with pBI121-containing Agrobacterium tumefaciens C58 produced transgenic calli, as confirmed by beta-glucuronidase (GUS) staining. The regeneration efficiency of independent transgenic plants was approximately 13%, as revealed by genomic Southern analysis. Some transgenic plants were produced in as little as 5 months after co-cultivation. This system may help to facilitate studies of gene functions in tree growth and development at a genome level.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Jingyuan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Xibeiwang, Haidian District, Beijing 100094, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Shanfa</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Zenn-Zong</ForeName><Initials>ZZ</Initials></Author><Author ValidYN="Y"><LastName>Lourenco</LastName><ForeName>Rodrigo</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Chiang</LastName><ForeName>Vincent L</ForeName><Initials>VL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2006</Year><Month>10</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>Plant Cell Physiol</MedlineTA><NlmUniqueID>9430925</NlmUniqueID><ISSNLinking>0032-0781</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018745" MajorTopicYN="N">Genome, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D023281" MajorTopicYN="N">Genomics</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005838" MajorTopicYN="N">Genotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005966" MajorTopicYN="N">Glucuronidase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012038" MajorTopicYN="N">Regeneration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="Y">Transformation, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>10</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>2</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>10</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17018558</ArticleId><ArticleId IdType="pii">pcl018</ArticleId><ArticleId IdType="doi">10.1093/pcp/pcl018</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Chen, Zenn Zong" sort="Chen, Zenn Zong" uniqKey="Chen Z" first="Zenn-Zong" last="Chen">Zenn-Zong Chen</name><name sortKey="Chiang, Vincent L" sort="Chiang, Vincent L" uniqKey="Chiang V" first="Vincent L" last="Chiang">Vincent L. Chiang</name><name sortKey="Lourenco, Rodrigo" sort="Lourenco, Rodrigo" uniqKey="Lourenco R" first="Rodrigo" last="Lourenco">Rodrigo Lourenco</name><name sortKey="Lu, Shanfa" sort="Lu, Shanfa" uniqKey="Lu S" first="Shanfa" last="Lu">Shanfa Lu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Song, Jingyuan" sort="Song, Jingyuan" uniqKey="Song J" first="Jingyuan" last="Song">Jingyuan Song</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003E12 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003E12 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:17018558
|texte= Genetic transformation of Populus trichocarpa genotype Nisqually-1: a functional genomic tool for woody plants.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:17018558" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |